John J. Peluso
Professor
Department of Cell
Biology
University of Connecticut Health Center
263 Farmington Ave
Farmington, CT 06032
860 679 2860
860 679 1269 (fax)
email - peluso@nso2.uchc.edu
Research interests:
Hormonal control of ovarian follicular growth and atresia; cellular and molecular mechanisms involved in apoptosis; Characterization of a novel membrane receptor for progesterone (PGRMC1) including the elucidation of its signal transduction pathway. Studies included identification of proteins that bind PGRMC1 using proteomics, protein-protein interaction assays and genetic approaches to deplete or enhance expression. Additional studies are being conducted on PGRMC1's role in modulating the sensitivity of ovarian cancer cells to chemotherapy both in vitro and in vivo.
Expression and structural features of a novel membrane
receptor for progesterone (PGRMC1). The panel at the left shows the different
domains of PGRMC1. However, the proteins that bind PGRMC1 and are presumably
involved in ligand activated signal transduction have not been identified.
The panel at the right shows the expression of PGRMC1 within the immature
rat ovary.
Recent
publications
Peluso, J.J., Liu, X., Gawkowska, A., and Johnston-MacAnanny, E. 2009. Progesterone activates a progesterone receptor membrane component-1-dependent mechanism that promotes human granulose / luteal cell survival but not progesterone secretion. J. Clinical Endocrinology and Metabolism 94: 2644-2649.
Peluso, J.J., Gawkowska, A., Liu, X., Shioda, T., and Pru, J.K. 2009. Progesterone receptor membrane component-1 regulates the development and cisplatin sensitivity of human ovarian tumors in athymic nude mice. Endocrinology (in press).
Mansouri, M, Schuster, J, Badhai, J, Stattin E-L, Losel, R, Wheling, M, Carlsson, B, Hovatta, O, Karlstrom, PO, Golovleva, I, Toniolo, D, Bione, S, Peluso, JJ, Dahl, N: Premature ovarian failure associated with alterations in the expression, structure and function of progesterone receptor membrane componenet-1 (PGRMC1). Hum Mol Gen 17: 3776-3783, 2008.
Losel, RM, Besong, D, Peluso, JJ and Mehling, M: Progesterone receptor membrane component-1 – many tasks for a versatile protein. Steroids 73: 929-934, 2008
Peluso, JJ, Liu, X, Saunders, MM, Claffey, KP, Phoenix, K: Regulation of ovarian cancer cell viability and sensitivity to cisplatin by progesterone receptor membrane component-1 J Clin Endocr Met 93: 1593-1599, 2008.
Wu, CA, Peluso, JJ, Shanley, JD, Puddington, L, Thrall, RS: Murine cytomegalovirus influences Foxj1 expression, ciliogenesis and mucus plugging in mice with allergic airway disease. Am J Path 172: 714-724, 2008.
Zhang, L, Kanda, Y, Roberts, DJ, Ecker, JL, Losel, R, Wehling, M, Peluso, JJ and Pru, JK: Expression of Progesterone Membrane Component 1 and Its Binding Partner Serpine 1 mRNA Binding Protein in Uterine and Placental Tissues of the Mouse and Human. Mol Cell Endo 287: 81-89, 2008.
Peluso, JJ, Romak, JJ and Liu, X: Progesterone Receptor Membrane Component-1 (PGRMC1) is the Mediator of Progesterone’s Anti-apoptotic Action in Spontaneously Immortalized Granulosa Cells as Revealed by PGRMC1 siRNA Treatment and Functional Analysis of PGRMC1 Mutations. Endocrinology 149: 534-543, 2008.
