Department of Cell Biology
263 Farmington Avenue
Farmington, CT 06030
Structure and function of the endoplasmic reticulum; serial section electron microscopy; plasma membrane re-sealing; mechanism of nuclear envelope breakdown.
Lab Member's page See posters of current work!
Terasaki, M., Shemesh, T., Kasthuri, N., Klemm, R.W., Schalek, R., Hayworth, K.J., Hand, A.R., Yankova, M., Huber, G., Lichtman, J.W., Rapoport, T., and Kozlov, M.M. 2013. Stacked endoplasmic reticulum sheets are connected by helicoidal membrane motifs. Cell 154: 285-296. pdf See also Cell Preview: Marshall, W.F. 2013. Differential geometry meets the cell. Cell 154: 265-266. pdf
This paper explains how parallel sheets of endoplasmic reticulum are connected. The existence of these sheet stacks and their function in synthesis of membrane and secreted proteins has been known for more than 60 years. Three-dimensional reconstructions from serial section electron micrographs from the mouse salivary gland show that the sheets are connected by twisted membrane surfaces with helical edges. The overall structure resembles a parking garage, in which the different levels are connected by helicoidal ramps. Theoretical calculations show that this structure minimizes the elastic energy of sheet edges and surfaces and allows dense packing of this organelle in the restricted space of a cell.
Green, S.A., Norris, R.P., Terasaki, M., Lowe, C.J. 2013. FGF signaling induces mesoderm in the hemichordate Saccoglossus kowalevskii. Development 140: 1024-1033. pdf
Darras, S., Gerhart, J., Terasaki, M., Kirschner, M., Lowe, C.J. 2011. B-Catenin specifies the endomesoderm and defines the posterior organizer of the hemichordate Saccoglossus kowalevskii. Development 138, 959-970. pdf
Terasaki, M., and Runft, L. 2010. Two-stage dependence for 1-methyladenine induced reinitiation of meiotic maturation in starfish oocytes. Exp. Cell Res. 316: 2654-2663. pdf
Lowe, C.J., Terasaki, M., Wu, M., Freeman, R., Runft, L., Kwan, K., Haigo, S., Aronowicz, J., Lander, E., Gruber, C., Kirschner, M., and Gerhart, J. 2006. Dorsoventral patterning in hemichordates: insights into early chordate evolution. PLOS 4: 1603-1619. pdf
Terasaki, M. 2006. Fluorescence quantitation in thick specimens, with an application to cyclin B-GFP expression in starfish oocytes. Biol. Cell 98: 245-252. pdf
Fein, A., and Terasaki, M. 2005. Rapid increase in plasma membrane chloride permeability during wound resealing in starfish oocytes. J. Gen. Physiol. 126: 151-159 pdf
Lenart, P., Daigle, N., Bacher, C., Hand, A.R. , Eils, R., Terasaki, M., and Ellenberg, J. 2005. A contractile nuclear actin network drives chromosome congression in oocytes. Nature 436: 812-818 pdf
Terasaki, M., Okumura, E., Hinkle, B., and Kishimoto, T. 2003. Localization and dynamics of Cdc2-cyclin B during meiotic reinitation in starfish oocytes. Mol. Biol. Cell 14:4685-4694. pdf
Slepchenko, B.M., and Terasaki, M. 2003. Cyclin aggregation and robustness of bio-switching. Mol. Biol. Cell 14: 4695-4706. pdf
Galbraith, J.A., and Terasaki, M. 2003. Controlled damage in thick specimens by multiphoton excitation. Mol. Biol. Cell 14: 1808-1817. pdf
Hinkle, B., Slepchenko, B., Rolls, M.M., Walther, T.C., Stein, P.A., Mehlmann, L.M., Ellenberg, J., and Terasaki, M. 2002. Chromosomal association of Ran during meiotic and mitotic divisions. J. Cell Sci. 115: 4685-4693. pdf
Terasaki, M., Runft, L., and Hand, A. 2001. Changes in organization of the endoplasmic reticulum during Xenopus oocyte maturation and egg activation. Mol. Biol. Cell 12: 1103-1116. pdf
Terasaki, M., Campagnola, P., Rolls, M.M., Stein, P., Ellenberg, J., Hinkle, B., and Slepchenko, B. 2001. A new model for nuclear envelope breakdown. Mol. Biol. Cell 12: 503-510. pdf